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A few plasmids (pGL1124, pGL1224 and pGL1217) had been constructed to allow the substitution of one allele of CYC9To research the subcellular localization of your CRK12 protein, a pEarleyGate104 vector was used for a transient expression of your CRK12 protein fused to yellow fluorescent protein (YFP). The confocal photos with the P. vulgarisRNAi. C